کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10757012 | 1050392 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Generation of stable 3â²-mRNA cleavage fragments induced by siRNA in cells with high-levels of duck hepatitis B virus replication
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کلمات کلیدی
shRNAASOSDHBVAntisense oligodeoxynucleotides - oligodeoxynucleotide antisenseSmall interfering RNA - RNA تداخل کوچکshort hairpin RNA - RNA موی سر کوتاهsiRNA - siRNASingle stranded - تک رشتهRISC - خطرSide effect - عوارض جانبیRNA-Induced Silencing Complex - مجتمع خاموش کننده RNA inducedMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNADuck hepatitis B virus - ویروس هپاتیت B رقم اردکpol - پلPolymerase - پلیمراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Generation of stable 3â²-mRNA cleavage fragments induced by siRNA in cells with high-levels of duck hepatitis B virus replication Generation of stable 3â²-mRNA cleavage fragments induced by siRNA in cells with high-levels of duck hepatitis B virus replication](/preview/png/10757012.png)
چکیده انگلیسی
Therapeutic small interfering RNAs (siRNAs) have attracted a lot of interest both in basic biomedical sciences as well as in translational medicine. Apart from their therapeutic efficacy adverse effects of siRNAs must be addressed. The generation of stable mRNA cleavage fragments and the translation of N-truncated proteins induced by antisense oligodeoxynucleotides (ASOs) have been reported. Similar to ASOs, siRNAs are considered to function via an antisense mechanism that promotes the cleavage of the target mRNA. To further investigate whether the stable mRNA cleavage fragments also occur in siRNA we constructed a short hairpin RNA (shRNA) expression plasmid, pshRNA794, containing the same sequence reported in experiments using ASOs which directly targeted the overlapping region of the pre-genomic mRNA (pgmRNA) and sub-genomic mRNA (sgmRNA) of duck hepatitis B virus (DHBV). The shRNA resulted in a 70.9% and 69.9% reduction of the DHBV mRNAs in LMH and HuH-7 cells, respectively. In addition a 70% inhibition of the DHBV DNA level was observed. Interestingly, 3â²-mRNA cleavage fragments were detected in LMH but not in HuH-7 cells. Taken together, our findings demonstrate that the ASO sequence was also effective in siRNA. Importantly, our results provide direct evidence that stable 3â²-mRNA fragments were generated by siRNA in cells with high levels of DHBV replication. Whether these can cause adverse RNAi effects needs to be explored further.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 443, Issue 3, 17 January 2014, Pages 834-839
Journal: Biochemical and Biophysical Research Communications - Volume 443, Issue 3, 17 January 2014, Pages 834-839
نویسندگان
Lin Lan, Qing Mao, Hubert E. Blum,