کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10757452 | 1050394 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of vegetative storage protein (VSP) and low molecular proteins induced by water deficit in stolon of white clover
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کلمات کلیدی
2-DENDPKLTPSROS - ROSSDS–PAGE - SDS-PAGEVsp - VSPTwo-dimensional electrophoresis - الکتروفورز دو بعدیsodium dodecylsulfate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اکریللید سدیم دودسیل سولفاتNucleoside diphosphate kinase - دی اکسید کیناز دی اکسید کربنWhite clover - شبدر سفیدMALDI-TOF-MS - مالدی TOF-MSProteomics - پروتئومیکسvegetative storage protein - پروتئین ذخیره سازی گیاهیLipid transfer proteins - پروتئین های انتقال لیپیدWater-deficit - کمبود آبReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In stolon of white clover (Trifolium repens L.), the 17.3Â kDa protein has been newly identified as a vegetative storage protein (VSP) which has preponderant roles in N accumulation and mobilization to sustain growth when capacity of N uptake is strongly reduced. To characterize the water deficit effect on this protein, the kinetic pattern of soluble protein, SDS-PAGE, Western blotting, and proteomic analysis was studied in the stolon of white clover during 28Â days of water-deficit. Water deficit led to decrease protein concentration. SDS-PAGE revealed that two major proteins of 17.3 and 16Â kDa were accumulated to high level in response to water stress. These proteins cross-reacted positively with antibodies raised against the 17.3Â kDa VSP, a protein which shared biochemical features with stress proteins implied in dehydration tolerance. Using two-dimensional electrophoresis (2-DE) gel and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF-MS) analysis, it was demonstrated that 19.5 and 17.3Â kDa protein spots were up-regulated by water stress, and both spots were identical to nucleoside diphosphate kinase (NDPK) and lipid transfer proteins (LTPs), respectively. These results suggest that low molecular proteins induced by water-deficit in the stolon of white clover act as an alternative N reserves or play significant roles in plant protection against water-deficit stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 443, Issue 1, 3 January 2014, Pages 229-233
Journal: Biochemical and Biophysical Research Communications - Volume 443, Issue 1, 3 January 2014, Pages 229-233
نویسندگان
Bok-Rye Lee, Dong-Gi Lee, Jean-Christophe Avice, Tae-Hwan Kim,