کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10758167 | 1050404 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
POT1b regulates phagocytosis and NO production by modulating activity of the small GTPase Rab5
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کلمات کلیدی
PBSGFPNOSiNOSEEA1Rab5inducible NOS - NOS قابل القاییearly endosome antigen 1 - آنتیژن آندوسوم اولیه 1inflammation - التهاب( توروم) GAP - شکافPhagocytosis - فاگوسیتوز یا بیگانهخواری Phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریNitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازGTPase-activating protein - پروتئین فعال GTPaseGreen fluorescence protein - پروتئین فلورسانس سبز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The protection of telomeres 1 (POT1) protein is a 75-kDa protein that plays an important role in telomere protection, which is related to telomere elongation. Although POT1 is present in and acts in the nuclei, little is known about the functions of POT1 in the cytosol. We here examined the role of POT1b in phagocytosis in a macrophage-like RAW 264 cell line. We found that POT1 was present in the cytosol, where it was bound to Rab5, which is a protein important for endocytosis. POT1b knockdown in RAW 264 cells increased Rab5 activity and facilitated the phagocytosis of whole cells of Escherichia coli and Staphylococcus aureus. Furthermore, POT1b knockdown enhanced the expression of inducible nitric oxide synthase (iNOS), followed by the promotion of nitric oxide (NO) generation in response to stimulation by bacterial whole cells. These results suggest that POT1b negatively regulates phagocytosis by controlling Rab5 activity and thereby modulates bacteria-induced NO generation. These findings suggest that POT1b participates in innate immune responses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 439, Issue 3, 27 September 2013, Pages 413-417
Journal: Biochemical and Biophysical Research Communications - Volume 439, Issue 3, 27 September 2013, Pages 413-417
نویسندگان
Makoto Hagiwara, Toshinori Komatsu, Shin-suke Sugiura, Ryutaro Isoda, Hiroyuki Tada, Naomi Tanigawa, Yoshiko Kato, Naoyuki Ishida, Kaoru Kobayashi, Kenji Matsushita,