کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10758442 | 1050407 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CTLA-4Ig immunotherapy of obesity-induced insulin resistance by manipulation of macrophage polarization in adipose tissues
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کلمات کلیدی
SVFMCPNCDHFDAUC - AUCRheumatoid arthritis - آرتریتروماتوئیدinsulin tolerance test - آزمون تحمل انسولینintraperitoneal glucose tolerance test - آزمون تحمل گلوکز داخل صفاقیITT - اینجاHigh fat diet - رژیم غذایی با چربی بالاnormal chow diet - رژیم غذایی معمول چوAnti-inflammation - ضد التهابMacrophage polarization - قطبش ماکروفاژObesity - مرض چاقیInsulin resistance - مقاومت به انسولینarea under the curve - منطقه تحت منحنیAIM - هدفmonocyte chemotactic protein - پروتئین chemotactic monocyteStromal vascular fraction - کسر عروق استروما
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
It has been established that obesity alters the metabolic and endocrine function of adipose tissue and, together with accumulation of adipose tissue macrophages, contributes to insulin resistance. Although numerous studies have reported that shifting the polarization of macrophages from M1 to M2 can alleviate adipose tissue inflammation, manipulation of macrophage polarization has not been considered as a specific therapy. Here, we determined whether cytotoxic T-lymphocyte-associated antigen-4IgG1 (CTLA-4Ig) can ameliorate insulin resistance by induction of macrophages from proinflammatory M1 to anti-inflammatory M2 polarization in the adipose tissues of high fat diet-induced insulin-resistant mice. CTLA4-Ig treatment prevented insulin resistance by changing gene expression to M2 polarization, which increased the levels of arginase 1. Furthermore, flow cytometric analysis confirmed the alteration of polarization from CD11c (M1)- to CD206 (M2)-positive cells. Concomitantly, CTLA-4Ig treatment resulted in weight reductions of epididymal and subcutaneous adipose tissues, which may be closely related to overexpression of apoptosis inhibitors in macrophages. Moreover, proinflammatory cytokine and chemokine levels decreased significantly. In contrast, CCAAT enhancer binding protein α, peroxisome proliferator-activated receptor γ, and adiponectin expression increased significantly in subcutaneous adipose tissue. This novel mechanism of CTLA-4lg immunotherapy may lead to an ideal anti-obesity/inflammation/insulin resistance agent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 438, Issue 1, 16 August 2013, Pages 103-109
Journal: Biochemical and Biophysical Research Communications - Volume 438, Issue 1, 16 August 2013, Pages 103-109
نویسندگان
Masakazu Fujii, Toyoshi Inoguchi, Battsetseg Batchuluun, Naonobu Sugiyama, Kunihisa Kobayashi, Noriyuki Sonoda, Ryoichi Takayanagi,