کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10759265 | 1050417 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer
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کلمات کلیدی
EGFRDMEMGAPDHH3K27lincRNAcDNA - cDNAComplementary DNA - DNA تکمیلیEMT - تکنسین فوریتهای پزشکیDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده Dulbeccohematoxylin eosin - هماتوکسیلین ائوزینEpithelial-mesenchymal transition - گذار اپیتلیال-مزانشیمیglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژنازEpidermal growth factor receptor - گیرنده فاکتور رشد اپیدرمال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
HOTAIR is one of long non-coding RNAs and its expression correlates with the prognosis and metastasis in various cancers. We showed that HOTAIR expression has an important role in the development of non-small cell lung cancer (NSCLC). In this study, we examined the expression of HOTAIR in 77 NSCLCs, their corresponding normal lung tissues and 6 brain metastases by quantitative real-time RT-PCR. High expression of HOTAIR (tumor/normal ratio ⩾2) was detected in 17 patients (22.1%) and was frequently found in patients with advanced stage, lymph node metastasis or lymph-vascular invasion and short disease free interval. Furthermore, brain metastases show significantly higher HOTAIR expression compared to primary cancer tissues. HOTAIR-expressing A549 cells showed induced cell migration and anchorage-independent cell growth in vitro. These results indicate the expression of HOTAIR enhanced the aggressive behavior of NSCLC cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 436, Issue 2, 28 June 2013, Pages 319-324
Journal: Biochemical and Biophysical Research Communications - Volume 436, Issue 2, 28 June 2013, Pages 319-324
نویسندگان
Takayuki Nakagawa, Hiroyuki Endo, Misa Yokoyama, Jiro Abe, Keiichi Tamai, Nobuyuki Tanaka, Ikuro Sato, Satomi Takahashi, Takashi Kondo, Kennichi Satoh,