Generation of reactive oxygen species by a novel berberine-bile acid analog mediates apoptosis in hepatocarcinoma SMMC-7721 cells

- Anticancer effects of B4, a novel berberine-bile acid analog, were tested.
- B4 inhibited cell proliferation in hepatocellular carcinoma cells.
- It also stimulated mitochondrial ROS production and membrane depolarization.
- Effects of B4 were inhibited by a non-specific ROS scavenger.
- Regulation of ROS generation may be a strategy for treating hepatic carcinoma.