Phospholipase C-related catalytically inactive protein, a novel microtubule-associated protein 1 light chain 3-binding protein, negatively regulates autophagosome formation

► PRIP is a novel negative modulator in nutrient depletion-induced autophagy. ► PRIP, a binding partner of GABARAP, interacts with an autophagy initiator, LC3. ► Enhanced PRIP colocalization with LC3 occurs in starved MEFs. ► PRIP deficiency increases autophagosome formation in starved MEFs. ► PRIP overexpression inhibits autophagosome formation in starved MEFs.