Closed-looped in situ nano processing on a culturing cell using an inverted electron beam lithography system

► An electron beam lithography (EBL) was used as an in situ nano processing for a living cell. ► A synchronized optics was containing an inverted EBL and an optical microscope. ► This system visualized real-time images of the EB-induced nano processing. ► We demonstrated the nano processing for a culturing cell with 200-300 nm resolution. ► Our system would be able to provide high resolution display of virtual environments.