Adhesin activity of Leptospira interrogans lipoprotein identified by in vivo and in vitro shotgun phage display

► LIC12976 protein was selected in vivo and in vitro shotgun phage display library. ► rLIC12976 binds to A31, LLC-PK1 and VERO cells and specifically to laminin. ► rLIC12976 binds more to mammalian cells in competition assay with laminin. ► lic12976 gene is conserved and expressed in pathogenic serovars.