Laminar shear stress enhances endothelial cell survival through a NADPH oxidase 2-dependent mechanism

► Laminar shear stress (LSS) prevented endothelial cell death due to serum depletion. ► Inhibition of NADPH oxidases (NOX) abrogated the pro-survival effect of LSS. ► The pro-survival effect of LSS was reduced by NOX2 siRNA, but not by NOX4 siRNA. ► LSS increased the expressions of p47phox and p67phox, the subunits of NOX2 complex.