Synergistic cytotoxic action of cisplatin and withaferin A on ovarian cancer cell lines

► Novel combination therapy using withaferin A (WFA) and cisplatin. ► Combination of WFA with cisplatin elicits synergistic cytotoxic effects. ► Combination of WFA with cisplatin significantly reduces the cisplatin dosage requirement. ► WFA induces ROS generation, whereas cisplatin induces DNA damage in combination treatment. ► Lower dose of cisplatin in combination therapy will minimize/eliminate side effects of cisplatin.