Ahnak1 interaction is affected by phosphorylation of Ser-296 on Cavβ2

► Mapping of Cavβ2 core region (SH3-HOOK-GK) as ahnak1 interacting domain. ► Identification of Ser-296 as a novel PKA phosphorylation site located in the GK domain of Cavβ2. ► Phosphorylation of Ser-296 on Cavβ2 influences its interaction with ahnak1.