Validation of trans-acting elements that promote exon 7 skipping of SMN2 in SMN2-GFP stable cell line

► GFP reporter stable cell lines that recapitulate exon 7 inclusion of SMN1 and SMN2 pre-mRNA are established. ► Alternative splicing of SMN1-GFP and SMN2-GFP mini-gene is similar to endogenous pre-mRNA splicing of SMN1 and SMN2 gene. ► SMN1-GFP stable cell line expresses ∼fourfold GFP protein than SMN2-GFP stable cell line does. ► shRNA knockdown of hnRNP A1 in SMN2-GFP stable cell line promotes exon 7 inclusion of SMN2-GFP minigene.