Lubiprostone activates CFTR, but not ClC-2, via the prostaglandin receptor (EP4)

► Lubiprostone chloride transport was examined in Xenopus oocytes. ► We studied ClC-2 and CFTR chloride channels. ► We examined both channels co-expressed with prostaglandin receptor sub-type 4. ► We demonstrate CFTR but not ClC-2 activation by lubiprostone in oocytes.