Increased stemness and migration of human mesenchymal stem cells in hypoxia is associated with altered integrin expression

► Hypoxic cell culture delays senescence of hMSC. ► Hypoxia increases homogeneity of hMSC and promotes rapidly self-renewing cells. ► Cell height and volume are reduced in hypoxic environment. ► Hypoxia affects migration of hMSCs on various surfaces. ► Integrin expression is altered in hypoxic hMSCs.