Gs cascade regulates canonical transient receptor potential 5 (TRPC5) through cAMP mediated intracellular Ca2+ release and ion channel trafficking

► β-AR stimulation increases basal TRPC5 current while inducing Ca2+ transients. ► Gs cascade increase basal TRPC5 current and this effect accompanies cytosolic Ca2+. ► β-AR stimulation as well as Gs cascade increases surface expression level of TRPC5. ► Gs cascade-IP3R-mediated Ca2+ release potentiates TRPC5.