Flt1 and Flk1 mediate regulation of intraocular pressure and their double heterozygosity causes the buphthalmia in mice

► Flt1+/−; Flk1+/− mice show the buphthalmia without hallmarks of glaucoma. ► Intraocular pressure is elevated in Flt1+/−, Flk1+/−, and Flt1+/−; Flk1+/− mice. ► Outflow pathway of the aqueous humor is affected in Flt1+/− and Flk1+/− mice.