Pan-neuronal knockdown of the c-Jun N-terminal Kinase (JNK) results in a reduction in sleep and longevity in Drosophila

► Pan-neuronal JNK knockdown reduces sleep and longevity in Drosophila. ► JNK heterozygous knockout flies show a reduction in sleep. ► JNK knockdown flies show a normal arousal threshold and rebound sleep. ► Lifespan of JNK knockdown is short, and even shorter on a low calorie diet. ► JNK knockdown in the mushroom body, not the pars intercerebralis reduces sleep.