MT1-MMP modulates the mechanosensitivity of osteocytes

► MT1-MMP knockdown increased the osteocyte response to mechanical stimulation. ► MT1-MMP knockdown increased the number and size of focal adhesions in osteocytes. ► MT1-MMP−/− mice had a lower number of empty lacunae and a higher osteocyte density. ► Mechanical stimulation decreased MT1-MMP expression by osteocytes. ► Our results show a novel and unexpected role for MT1-MMP in mechanosensing.