Vitamin D receptor controls expression of the anti-aging klotho gene in mouse and human renal cells

► Both klotho mRNA spliceforms are regulated by 1,25-dihydroxyvitamin D (1,25D). ► Regulation occurs in cell lines from kidney proximal, distal and collecting tubules. ► Candidate vitamin D response elements (VDREs) found near mouse and human kl genes. ► Two human VDREs and one mouse VDRE are active in reporter gene assays. ► Klotho regulation by 1,25D may potentiate phosphaturic effect of FGF23.