Monitoring succinyl-CoA:3-oxoacid CoA transferase nitration in mitochondria using monoclonal antibodies

► The generation of monoclonal antibodies by nitrated SCOT peptides as the antigens. ► The immuno-identification of nitrated Tyr4-SCOT and Tyr76-SCOT in vitro and in vivo. ► Monitoring the nitration status of SCOT in diabetic mouse tissues.