The cross-road between the mechanisms of protein folding and aggregation; study of human stefin B and its H75W mutant

► Protein folding and protein aggregation may have some common intermediate. ► Human stefin B is a suitable model system to study amyloidogenesis. ► His 75 to Trp mutation of the protein lead to differences in folding and amyloid formation. ► We predict that the mutant would form mature, regular fibrils at pH 7.0. ► At pH's lower than 6.0 it may aggregate into protofibrils.