Effects of cold exposure and shear stress on endothelial nitric oxide synthase activation

► The role of endothelial cells (ECs) in blood vessel regulation in response to cold temperatures is not clear. ► We investigated whether low temperature changes eNOS activations inside ECs. ► We found that low temperature alone did not change eNOS activation in ECs. ► However, the combination of shear stress with cold temperature does decrease eNOS activation.