Genistein promotes DNA demethylation of the steroidogenic factor 1 (SF-1) promoter in endometrial stromal cells

► Genistein (GEN) is a phytoestrogen found in soy products. ► GEN demethylated/unsilenced the steroidogenic factor 1 gene in endometrial tissue. ► GEN thus altered mRNA expression in uteri of ovariectomized (OVX) mice. ► A high-resolution melting assay was used to screen for epigenetic change. ► We isolated an endometrial cell clone that was epigenetically modulated by GEN.