Development of recombinant Aleuria aurantia lectins with altered binding specificities to fucosylated glycans

► Linkage specific changes in fucosylation have been associated with cancer. ► The Aleuria aurantia lectin (AAL) can bind fucose but has limited linkage specificity. ► Through site-directed mutagenesis we developed AALs with altered binding affinities. ► These AAL molecules could be used to determine fucose changes in cancer.