A novel tripolymer coating demonstrating the synergistic effect of chitosan, collagen type 1 and hyaluronic acid on osteogenic differentiation of human bone marrow derived mesenchymal stem cells

► Chitosan-collagen-hyaluronic acid tripolymer coating enhanced mineralization. ► Chitosan-collagen (1:1) plate layered with hyaluronic acid had highest calcium. ► Surface modification method of coating is more effective than polyblend method. ► Mineralization is stringently controlled by the mixing ratio and method of coating. ► A prospective biomimetic surface coating to improve osteoconductivity of implants.