Matrix vesicles isolated from mineralization-competent Saos-2 cells are selectively enriched with annexins and S100 proteins

► Functional MVs of Saos-2 cells are enriched in annexins A1, A2, A6 and A7. ► Some annexin levels in MVs change upon stimulation for mineralization. ► Annexin A6 is EGTA-non-extractable from MVs of Saos-2 cells. ► S100A10 and S100A6, but not S100A11, are selectively translocated to MVs.