Functional dissection of the Hox protein Abdominal-B in Drosophila cell culture

► ct340 CRM was identified to be the posterior spiracle enhancer of gene cut. ► ct340 is under the direct transcriptional control of Hox protein Abd-B. ► An efficient cloning system was developed to assay protein-DNA interaction. ► New features of Abd-B dependent target gene regulation were detected.