Effects on the conformation of FVIIa by sTF and Ca2+ binding: Studies of fluorescence resonance energy transfer and quenching

► We have for the first time managed to estimate the length of FVIIa by FRET analysis. ► The global average conformation of FVIIa is nearly unaltered upon ligation to sTF. ► We have directly shown conformational changes in the active site upon Ca2+ binding.