Longistatin, a novel plasminogen activator from vector ticks, is resistant to plasminogen activator inhibitor-1

► Longistatin is less susceptible to SDS-treated platelet lysate. ► Longistatin retained 76.73% activity after 3 h of incubation with 20 nM of PAI-1. ► Longistatin lysed fibrin clot by activating plasminogen in the presence of PAI-1. ► Unlike t-PA, longistatin did not form complex with PAI-1. ► Longistatin is a PAI-1 resistant plasminogen activator.