The dynamic equilibrium between ATP synthesis and ATP consumption is lower in isolated mitochondria from myotubes established from type 2 diabetic subjects compared to lean control

► Mitochondrial ATP levels were evaluated at equilibrium between ATP synthesis and ATP utilization. ► Lower ATP levels at equilibrium in diabetic versus lean mitochondria isolated from myotubes. ► Mitochondrial dysfunction in type 2 diabetes in vivo is partly based on a primarily impaired ATP synthesis.