Increased number of Arginine-based salt bridges contributes to the thermotolerance of thermotolerant acetic acid bacteria, Acetobacter tropicalis SKU1100

► Comparative genomics of thermotolerant species showed an increased Lys to Arg change. ► Modeling study showed Lys to Arg substitutions occur in salt bridge formation. ► Increased number of Lys to Arg contributes to the thermotolerance of Acetobacter tropicalis.