The VEGFR2 receptor tyrosine kinase undergoes constitutive endosome-to-plasma membrane recycling

► VEGFR2 receptor tyrosine kinase undergoes constitutive recycling between the plasma membrane and endosomes. ► VEGFR2-EGFP expression in non-vascular cells resembles endogenous VEGFR2 distribution in vascular cells. ► VEGFR2-EGFP mobility is reduced by ∼30% upon ligand stimulation. ► TIRF microscopy shows VEGFR2-EGFP recycling rapidly between the plasma membrane and peripheral endosomes.