Cloning, expression and purification of the CCN family of proteins in Escherichia coli

► CCN proteins exert their roles via interactions with a variety of signalling proteins. ► They share a related segmented organisation of four domains. ► Each domain confer defined function/s of the protein. ► So far no structural data on any member of the CCN family due to high cysteine content. ► Here we report expression of pure CCN proteins paving the way for future studies.