In vitro enhanced differentiation of neural networks in ES gut-like organ from mouse ES cells by a 5-HT4-receptor activation

► From mouse embryonic stem cells, gut-like organ (ES gut) is differentiated. ► In ES guts, serotonin 4 receptor agonists can differentiate enteric neurons. ► This ES gut has enteric neurons and c-kit positive cells (interstitial cells of Cajal). ► This ES gut express peristaltic movements.