Crystal structures of human secretory proteins ZG16p and ZG16b reveal a Jacalin-related β-prism fold

► This is the first crystal structure of mammalian β-prism fold lectin, ZG16p. ► The crystal structure of ZG16b, a paralog of ZG16p, has also been solved to be β-prism fold. ► Glycerol binding and surface electric potential of ZG16p account for its binding preference towards mannose/glucose-type glycans and glycosaminoglycans.