Extrusion of the C-terminal helix in navel orangeworm moth pheromone-binding protein (AtraPBP1) controls pheromone binding

► pH-dependent pheromone binding to moth pheromone-binding protein (AtraPBP1). ► C-terminal helix blocks pheromone binding at low pH. ► C-terminal deletion promotes pheromone binding at low pH. ► H80A and H95A mutants promote pheromone binding at low pH. ► Extrusion of C-terminal helix controls pH-dependent pheromone binding.