کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10764845 | 1050567 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Human selenophosphate synthetase 1 has five splice variants with unique interactions, subcellular localizations and expression patterns
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کلمات کلیدی
FACSFBSSPSSECSECShaemagglutinin - hemagglutininROS - ROSAlternative splicing - جابجایی جایگزینfetal bovine serum - سرم جنین گاوSelenophosphate synthetase - سلنفسفات سنتتازSelenocysteine - سلنوسیستینSelenium - سلنیومPropidium iodide - پروتئین یدیدCell cycle - چرخه سلولیReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Selenophosphate synthetase 1 (SPS1) is an essential cellular gene in higher eukaryotes. Five alternative splice variants of human SPS1 (major type, ÎE2, ÎE8, +E9, +E9a) were identified wherein +E9 and +E9a make the same protein. The major type was localized in both the nuclear and plasma membranes, and the others in the cytoplasm. All variants form homodimers, and in addition, the major type forms a heterodimer with ÎE2, and ÎE8 with +E9. The level of expression of each splice variant was different in various cell lines. The expression of each alternative splice variant was regulated during the cell cycle. The levels of the major type and ÎE8 were gradually increased until G2/M phase and then gradually decreased. ÎE2 expression peaked at mid-S phase and then gradually decreased. However, +E9/+E9a expression decreased gradually after cell cycle arrest. The possible involvement of SPS1 splice variants in cell cycle regulation is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 397, Issue 1, 18 June 2010, Pages 53-58
Journal: Biochemical and Biophysical Research Communications - Volume 397, Issue 1, 18 June 2010, Pages 53-58
نویسندگان
Jin Young Kim, Kwang Hee Lee, Myoung Sup Shim, Hyein Shin, Xue-Ming Xu, Bradley A. Carlson, Dolph L. Hatfield, Byeong Jae Lee,