Functional reconstitution of monomeric CYP3A4 with multiple cytochrome P450 reductase molecules in Nanodiscs

► Human drug metabolizing cytochrome P450 CYP3A4 is functionally incorporated with its reductase in nanoscale bilayers. ► Maximal catalytic activity of CYP3A4 requires multiple reductase molecules. ► Facile system for high throughput screening of monomeric P450 activity described.