Nogo-B mediates HeLa cell adhesion and motility through binding of Fibulin-5

► Nogo-B binds to Fibulin-5 in vitro and in vivo. ► Overexpressing Nogo-B elevates the extracellular Fibulin-5. ► Nogo-B transports Fibulin-5 from the ER to the plasma membrane. ► Overexpressing Nogo-B alters HeLa cell motility.