کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10765107 | 1050589 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Calneuron I inhibits Ca2+ channel activity in bovine chromaffin cells
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کلمات کلیدی
BESDAPIHBSSN-methyl-d-glucamineCaBPNMG4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولcalcium current - جریان کلسیمCAM - ساخت به کمک کامپیوترCalcium signaling - سیگنالینگ کلسیمHank’s balanced salt solution - محلول نمک متعادل هانکEF-hand motif - موتیف EFCalcium binding protein - پروتئین اتصال دهنده کلسیمCalmodulin - کالمودولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Calneuron I (CalnI) is a calmodulin-like protein that contains two functional EF-hand motifs at the N-terminal and a hydrophobic segment at the C-terminal. CalnI was cloned from the adult rat cortex and fused with GFP at its N-terminal. When expressed in bovine chromaffin cells, wild-type CalnI was localized at the plasma membrane. However, a mutant that lacked the hydrophobic segment was localized in the cytosol and nucleus, while a Ca2+-binding-deficient mutant was found in the cytosol and at the plasma membrane. Evaluation using the whole-cell patch-clamp technique revealed that Ca2+ currents were inhibited by both wild-type CalnI and the Ca2+-binding-deficient mutant. When the bovine N-type Ca2+ channel was expressed in 293T cells, Ca2+ currents were mostly inhibited by co-expression of CalnI, but not by the mutant without the hydrophobic tail. These results suggest that CalnI attenuates Ca2+ channel activity and that its subcellular localization is important for this effect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 388, Issue 3, 23 October 2009, Pages 549-553
Journal: Biochemical and Biophysical Research Communications - Volume 388, Issue 3, 23 October 2009, Pages 549-553
نویسندگان
Po-Yuan Shih, Chih-Lung Lin, Po-Wen Cheng, Jia-Hong Liao, Chien-Yuan Pan,