کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10765107 1050589 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Calneuron I inhibits Ca2+ channel activity in bovine chromaffin cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Calneuron I inhibits Ca2+ channel activity in bovine chromaffin cells
چکیده انگلیسی
Calneuron I (CalnI) is a calmodulin-like protein that contains two functional EF-hand motifs at the N-terminal and a hydrophobic segment at the C-terminal. CalnI was cloned from the adult rat cortex and fused with GFP at its N-terminal. When expressed in bovine chromaffin cells, wild-type CalnI was localized at the plasma membrane. However, a mutant that lacked the hydrophobic segment was localized in the cytosol and nucleus, while a Ca2+-binding-deficient mutant was found in the cytosol and at the plasma membrane. Evaluation using the whole-cell patch-clamp technique revealed that Ca2+ currents were inhibited by both wild-type CalnI and the Ca2+-binding-deficient mutant. When the bovine N-type Ca2+ channel was expressed in 293T cells, Ca2+ currents were mostly inhibited by co-expression of CalnI, but not by the mutant without the hydrophobic tail. These results suggest that CalnI attenuates Ca2+ channel activity and that its subcellular localization is important for this effect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 388, Issue 3, 23 October 2009, Pages 549-553
نویسندگان
, , , , ,