کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10765273 | 1050592 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Establishment and characterization of a unique 1 μm diameter liver-derived progenitor cell line
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کلمات کلیدی
RT-PCRLDPCSV40 TagTem - این استCell line - خط تلفنretinoblastoma - رتینوبلاستوما، تومور شبکیهCancer - سرطانStem cell - سلول بنیادیTransmission electron microscopy - میکروسکوپ الکترونی عبوریreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسsimian virus 40 large T antigen - ویروس سیویان 40 آنتی ژن T بزرگprogenitor - پیشگامLiver - کبد
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Liver-derived progenitor cells (LDPCs) are recently identified novel stem/progenitor cells from healthy, unmanipulated adult rat livers. They are distinct from other known liver stem/progenitor cells such as the oval cells. In this study, we have generated a LDPC cell line RA1 by overexpressing the simian virus 40 (SV40) large T antigen (TAg) in primary LDPCs. This cell line was propagated continuously for 55 passages in culture, after which it became senescent. Interestingly, following transformation with SV40 TAg, LDPCs decreased in size significantly and the propagating cells measured 1 μm in diameter. RA1 cells proliferated in vitro with a doubling time of 5-7 days, and expressed cell surface markers of LDPCs. In this report, we describe the characterization of this novel progenitor cell line that might serve as a valuable model to study liver cell functions and stem cell origin of liver cancers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 391, Issue 1, 1 January 2010, Pages 56-62
Journal: Biochemical and Biophysical Research Communications - Volume 391, Issue 1, 1 January 2010, Pages 56-62
نویسندگان
Rajagopal N. Aravalli, M. Behnan Sahin, Erik N.K. Cressman, Clifford J. Steer,