کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10765378 | 1050592 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning and functional characterization of a mouse gene upregulated by lipopolysaccharide treatment reveals alternative splicing
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کلمات کلیدی
FCMNF-κBLPSGNBFBSPRRsLBPPAMPMAPK - MAPKpathogen-associated molecular pattern - الگوی مولکولی وابسته به پاتوژنGram-negative bacterium - باکتری گرم منفیAlternative splicing - جابجایی جایگزینfetal bovine serum - سرم جنین گاوnuclear factor-κB - فاکتور هسته ای κBFlow cytometry - فلوسیتومتریlipopolysaccharide - لیپوپلی ساکاریدMolecular cloning - همسانه سازی مولکولی، کلونینگ مولکولیFunctional characterization - ویژگی های عملکردیLPS binding protein - پروتئین متصل LPSmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenpattern recognition receptors - گیرنده های تشخیص الگو
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Treatment of mouse cells with lipopolysaccharide (LPS) potently initiates an inflammatory response, but the underlying mechanisms are unclear. We therefore sought to characterize cDNA sequences of a new mouse LPS-responsive gene, and to evaluate the effects of MLrg. Full-length cDNAs were obtained from LPS-treated NIH3T3 cells. We report that the MLrg gene produces two alternative splice products (GenBank Accession Nos. DQ316984 and DQ320011), respectively, encoding MLrgW and MLrgS polypeptides. Both proteins contain zinc finger and leucine zipper domains and are thus potential regulators of transcription. Expression of MLrgW and MLrgS were robustly upregulated following LPS treatment, and the proteins were localized predominantly in the nuclear membrane and cytoplasm. In stable transfectants over-expressing MLrgW the proportion of cells in G1 phase was significantly reduced, while in cells over-expressing MLrgS the proportion of cells in G2 was significantly increased; both proteins are thus potential regulators of cell cycle progression. Upregulation of MLrgW and MLrgS may be an important component of the LPS inflammatory pathway and of the host response to infection with GNB.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 391, Issue 1, 1 January 2010, Pages 267-271
Journal: Biochemical and Biophysical Research Communications - Volume 391, Issue 1, 1 January 2010, Pages 267-271
نویسندگان
Kejun Du, Yaoming Chen, Zongming Dai, Yuan Bi, Tongjian Cai, Lichao Hou, Yubo Chai, Qinghe Song, Sumin Chen, Wenjing Luo, Jingyuan Chen,