کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10765565 | 1050593 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Neuroblastoma GOTO cells are hypersensitive to disruption of lipid rafts
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کلمات کلیدی
mAbMβCDGM2GangliosideMonoclonal antibody - آنتی بادی مونوکلونالbromo-deoxyuridine - برومو دزوویریدینBrdU - بروموداکسی اوریدینTerminal deoxynucleotidyl transferase dUTP nick end labeling - ترمینال deoxynucleotidyl transferase dUTP نام نهایی پایان نامهTUNEL - تونلApoptosis - خزان یاختهایRAFT - رفعتK562 cell - سلول K562Schwann cell - سلول شوانCyclodextrin - سیکلودکسترینmethyl-β-cyclodextrin - متیل-β-سیکلوکودکسترینNeuroblastoma - نوروبلاستوماPropidium iodide - پروتئین یدید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Neuroblastoma GOTO cells are hypersensitive to disruption of lipid rafts Neuroblastoma GOTO cells are hypersensitive to disruption of lipid rafts](/preview/png/10765565.png)
چکیده انگلیسی
GOTO cells, a neuroblastoma cell line retaining the ability to differentiate into neuronal or Schwann cells, were found to be rich in membrane rafts containing ganglioside GM2 and hypersensitive to lipid raft-disrupting methyl-β-cyclodextrin (MβCD); the GM2-rich rafts and sensitivity to MβCD were markedly diminished upon their differentiation into Schwann cells. We first raised a monoclonal antibody that specifically binds to GOTO cells but not to differentiated Schwann cells and determined its target antigen as ganglioside GM2, which was shown to be highly concentrated in lipid rafts by its colocalization with flotillin, a marker protein of rafts. Disturbance of normal structure of the lipid raft by depleting its major constituent, cholesterol, with MβCD resulted in acute apoptotic cell death of GOTO cells, but little effects were seen on differentiated Schwann cells. Until this study, GM2-rich rafts are poorly characterized and MβCD hypersensitivity, which may have clinical implications, has not been reported.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 389, Issue 1, 6 November 2009, Pages 122-127
Journal: Biochemical and Biophysical Research Communications - Volume 389, Issue 1, 6 November 2009, Pages 122-127
نویسندگان
Ryosaku Tomioka, Natsumi Minami, Ai Kushida, Shiho Horibe, Ippei Izumi, Akira Kato, Keiko Fukushima, Hiroko Ideo, Katsuko Yamashita, Shigehisa Hirose, Yuji Saito,