کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10765567 1050593 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Glucose enhances collectrin protein expression in insulin-producing MIN6 β cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Glucose enhances collectrin protein expression in insulin-producing MIN6 β cells
چکیده انگلیسی
Collectrin is a novel target gene of hepatocyte nuclear factor-1α in pancreatic β-cells and controls insulin exocytosis. Although glucose is known to stimulate the expression of genes of the insulin secretory pathway, there is no information on how glucose regulates collectrin expression. We investigated the effects of glucose on the expression of collectrin in MIN6 β-cell line. Glucose, in a dose-dependent manner, increased collectrin protein levels without changing collectrin mRNA levels and protein stability, indicating that glucose stimulation of collectrin protein expression is primarily mediated at a translational level. Although mannose and pyruvate also increased collectrin protein expression level, neither 2-deoxyglucose, mitochondrial fuels leucine and glutamate, sulphonylurea nor Ca2+ channel blockers, mimicked the effects of glucose. These data indicate the involvement of mitochondrial TCA cycle intermediates, distal to pyruvate, in the regulation of collectrin protein expression in β-cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 389, Issue 1, 6 November 2009, Pages 133-137
نویسندگان
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