کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10766593 | 1050655 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nanostructure and force spectroscopy analysis of human peripheral blood CD4+ T cells using atomic force microscopy
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Nanostructure and force spectroscopy analysis of human peripheral blood CD4+ T cells using atomic force microscopy Nanostructure and force spectroscopy analysis of human peripheral blood CD4+ T cells using atomic force microscopy](/preview/png/10766593.png)
چکیده انگلیسی
To date, nanoscale imaging of the morphological changes and adhesion force of CD4+ T cells during in vitro activation remains largely unreported. In this study, we used atomic force microscopy (AFM) to study the morphological changes and specific binding forces in resting and activated human peripheral blood CD4+ T cells. The AFM images revealed that the volume of activated CD4+ T cells increased and the ultrastructure of these cells also became complex. Using a functionalized AFM tip, the strength of the specific binding force of the CD4 antigen-antibody interaction was found to be approximately three times that of the unspecific force. The adhesion forces were not randomly distributed over the surface of a single activated CD4+ T cell, indicated that the CD4 molecules concentrated into nanodomains. The magnitude of the adhesion force of the CD4 antigen-antibody interaction did not change markedly with the activation time. Multiple bonds involved in the CD4 antigen-antibody interaction were measured at different activation times. These results suggest that the adhesion force involved in the CD4 antigen-antibody interaction is highly selective and of high affinity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 374, Issue 1, 12 September 2008, Pages 90-94
Journal: Biochemical and Biophysical Research Communications - Volume 374, Issue 1, 12 September 2008, Pages 90-94
نویسندگان
Mingqian Hu, Jiongkun Wang, Jiye Cai, Yangzhe Wu, Xiaoping Wang,