کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10766726 | 1050678 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enrichment of corneal epithelial stem/progenitor cells using cell surface markers, integrin α6 and CD71
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Enrichment of corneal epithelial stem/progenitor cells using cell surface markers, integrin α6 and CD71 Enrichment of corneal epithelial stem/progenitor cells using cell surface markers, integrin α6 and CD71](/preview/png/10766726.png)
چکیده انگلیسی
Corneal epithelial stem cells are believed to reside in the basal layer of the limbal epithelium, but no definitive cell surface markers have been identified. For keratinocytes, stem/progenitor cells are known to be enriched by cell surface markers, integrin α6 and CD71, as a minor subpopulation which shows high integrin α6 and low CD71 expressions (α6bri/CD71dim). In the present study, we investigated the possibility that corneal epithelial stem cells can be enriched by integrin α6 and CD71. The α6bri/CD71dim cells were separated by fluorescence-activated cell sorting, as a minor subpopulation of the limbal epithelial cells. They were enriched for relatively small cells, showing a higher clonogenic capacity and expression of stem cell markers, but a lower expression of differentiation markers, compared to other cell populations. The cells were localized immunohistochemically in the basal region of the limbal epithelium. These results indicate that the α6bri/CD71dim subpopulation enriched corneal epithelial stem cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 367, Issue 2, 7 March 2008, Pages 256-263
Journal: Biochemical and Biophysical Research Communications - Volume 367, Issue 2, 7 March 2008, Pages 256-263
نویسندگان
Ryuhei Hayashi, Masayuki Yamato, Teiko Saito, Tetsuro Oshima, Teruo Okano, Yasuo Tano, Kohji Nishida,