کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767046 | 1050696 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Depot-specific effects of fatty acids on lipid accumulation in children's adipocytes
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کلمات کلیدی
IPAHIFBSG3PDHHBBSPBSDHAPFFASDSDMEMIBMXAdipocyte - آدیپوسیتFree fatty acid - اسید چرب آزادOleate - اولیتهIsopropanol - ایزوپروپانول، ایزوپروپیل الکلdihydroxyacetone phosphate - دی هیدروکسی استون فسفاتVisceral - ذره ایsubcutaneous - زیر جلدیheat-inactivated fetal bovine serum - سرم گاوی جنین گرم منفی شدهpalmitate - طلاییPhosphate buffered saline - فسفات بافر شورHank’s balanced salt solution - محلول نمک متعادل هانکDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoNADH - نادانStandard deviation score - نمره استاندارد انحرافNile Red - نیل قرمزChildren - کودکانglycerol-3-phosphate dehydrogenase - گلیسرول 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Circulating concentrations of fatty acids are elevated in obesity, although their effect on regional fat deposition is relatively unexplored. With the increasing prevalence of childhood obesity, we aimed to investigate whether saturated and unsaturated fatty acids lead to differential lipid accumulation (LA) in children's subcutaneous and visceral adipocytes. To examine this, subcutaneous and peri-nephric pre-adipocytes, isolated from fat biopsies from 6 pre-pubertal children, were differentiated in vitro before being exposed to palmitate and/or oleate for 24 h. Lipid accumulation was then quantified by nile red staining. Palmitate significantly increased LA in visceral adipocytes at all doses ⩾188 μM (e.g. Palmitate 750 μM: +30.0%[8.2]; p < 0.01), whilst only a dose of 375 μM led to a significant, but smaller, increase in LA in subcutaneous adipocytes (Palmitate 375 μM: +13.0%[4.3]; p = 0.02). In contrast, oleate significantly increased LA in subcutaneous (Oleate 1000 μM: +36.3%[14.0]; p = 0.01), but not visceral (Oleate 1000 μM: +16.2%[9.6]; p = 0.25) adipocytes. These data suggest that saturated and unsaturated fatty acids may exert depot-specific effects on lipid accumulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 361, Issue 2, 21 September 2007, Pages 356-361
Journal: Biochemical and Biophysical Research Communications - Volume 361, Issue 2, 21 September 2007, Pages 356-361
نویسندگان
Matthew A. Sabin, Elizabeth C. Crowne, Claire E. Stewart, Linda P. Hunt, Stephen J. Turner, Gavin I. Welsh, Malcolm J. Grohmann, Jeff M. Holly, Julian P. Shield,