کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10767149 1050698 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Prostaglandin E2 inhibits tumor necrosis factor-alpha RNA through PKA type I
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Prostaglandin E2 inhibits tumor necrosis factor-alpha RNA through PKA type I
چکیده انگلیسی
Tumor necrosis factor-alpha (TNF-α) is a cytokine that may contribute to the pathogenesis of septic shock, rheumatoid arthritis, cancer, and diabetes. Prostaglandins endogenously produced by macrophages act in an autocrine fashion to limit TNF-α production. We investigated the timing and signaling pathway of prostaglandin-mediated inhibition of TNF-α production in Raw 264.7 and J774 macrophages. TNF-α mRNA levels were rapidly modulated by PGE2 or carbaprostacylin. PGE2 or carbaprostacyclin prevented and rapidly terminated on-going TNF-α gene transcription within 15 min of prostaglandin treatment. Selective activation of PKA type I, but not PKA type II or Epac, with chemical analogs of cAMP was sufficient to inhibit LPS-induced TNF-α mRNA levels. The mechanisms by which prostaglandins limit TNF-α mRNA levels may underlie endogenous regulatory mechanisms that limit inflammation, and may have important implications for understanding chronic inflammatory disease pathogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 366, Issue 1, 1 February 2008, Pages 104-109
نویسندگان
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