کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767169 | 1050698 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lysyl oxidase propeptide inhibits smooth muscle cell signaling and proliferation
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کلمات کلیدی
Atherosclerosis - آترواسکلروز(تصلب شریان)Immunohistochemistry - ایمونوهیستوشیمیrestenosis - تنفسCell proliferation - تکثیر سلولیVascular smooth muscle cells - سلول های عضلانی صاف عروقیCell signaling - سیگنالینگ سلولیLysyl oxidase - لیسییل اکسیدازExtracellular matrix - ماتریکس خارج سلولیMatrix metalloproteinase-9 - ماتریکس متالوپروتئیناز -9Western blots - وسترن بلاتMAP kinase - کیناز MAP
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Lysyl oxidase is required for the normal biosynthesis and maturation of collagen and elastin. It is expressed by vascular smooth muscle cells, and its increased expression has been previously found in atherosclerosis and in models of balloon angioplasty. The lysyl oxidase propeptide (LOX-PP) has more recently been found to have biological activity as a tumor suppressor, and it inhibits Erk1/2 Map kinase activation. We reasoned that LOX-PP may have functions in normal non-transformed cells. We, therefore, investigated its effects on smooth muscle cells, focusing on important biological processes mediated by Erk1/2-dependent signaling pathways including proliferation and matrix metalloproteinase-9 (MMP-9) expression. In addition, we investigated whether evidence for accumulation of LOX-PP could be found in vivo in a femoral artery injury model. Recombinant LOX-PP was expressed and purified, and was found to inhibit primary rat aorta smooth muscle cell proliferation and DNA synthesis by more than 50%. TNF-α-stimulated MMP-9 expression and Erk1/2 activation were both significantly inhibited by LOX-PP. Immunohistochemistry studies carried out with affinity purified anti-LOX-PP antibody showed that LOX-PP epitopes were expressed at elevated levels in vascular lesions of injured arteries. These novel data suggest that LOX-PP may provide a feedback control mechanism that serves to inhibit properties associated with the development of vascular pathology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 366, Issue 1, 1 February 2008, Pages 156-161
Journal: Biochemical and Biophysical Research Communications - Volume 366, Issue 1, 1 February 2008, Pages 156-161
نویسندگان
Paola A. Hurtado, Siddharth Vora, Siddika Selva Sume, Dan Yang, Cynthia St. Hilaire, Ying Guo, Amitha H. Palamakumbura, Barbara M. Schreiber, Katya Ravid, Philip C. Trackman,